产品描述

This Human SPARC ELISA Kit was designed for the quantitative measurement of Human SPARC protein in serum, plasma, tissue homogenates, urine. It is a Sandwich ELISA kit, its detection range is 62.5 ng/mL-4000 ng/mL and the sensitivity is 15.6 ng/mL.

别名

AA517111 ELISA Kit; Basement membrane protein 40 ELISA Kit; Basement-membrane protein 40 ELISA Kit; BM 40 ELISA Kit; BM-40 ELISA Kit; BM40 ELISA Kit; Cysteine rich protein ELISA Kit; hm:zeh0062 ELISA Kit; MGC128090 ELISA Kit; OI17 ELISA Kit; ON ELISA Kit; Osteonectin ELISA Kit; Secreted acidic cystein rich glycoprotein ELISA Kit; Secreted protein acidic and cysteine rich ELISA Kit; Secreted protein acidic and rich in cysteine ELISA Kit; Secreted protein acidic cysteine rich (osteonectin) ELISA Kit; Secreted protein acidic cysteine rich ELISA Kit; SPARC ELISA Kit; SPRC ELISA Kit; SPRC_HUMAN ELISA Kit

缩写

SPARC

Uniprot No

P09486

灵敏度

15.6 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SPARC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		81-89
1:2		Average %		93
		Range %		89-97
1:4		Average %		98
		Range %		94-102
1:8		Average %		100
		Range %		95-105

回收率

The recovery of human SPARC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		87-93
EDTA plasma (n=4)		101		97-104

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 4000 2.120 2.301 2.211 2.049 2000 1.667 1.642 1.655 1.493 1000 1.173 1.163 1.168 1.006 500 0.759 0.787 0.773 0.611 250 0.453 0.469 0.461 0.299 125 0.307 0.301 0.304 0.142 62.5 0.254 0.259 0.257 0.095 0 0.163 0.160 0.162

货期

3-5 working days