产品描述
This Fish TNF-α ELISA Kit was designed for the quantitative measurement of Fish TNF-α protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 125 pg/mL-5000 pg/mL and the sensitivity is 125 pg/mL.
缩写
TNF-α
Uniprot No
Q8JFG3
灵敏度
125 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of fish TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
89-100 |
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| 1:2 |
Average % |
93 |
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| Range % |
87-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
88-103 |
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| 1:8 |
Average % |
96 |
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| Range % |
90-102 |
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回收率
| The recovery of fish TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
95-102 |
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| EDTA plasma (n=4) |
95 |
91-101 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 5000 |
0.154 |
0.156 |
0.155 |
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| 2500 |
0.351 |
0.328 |
0.340 |
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| 1150 |
0.562 |
0.535 |
0.549 |
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| 375 |
0.861 |
0.864 |
0.863 |
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| 125 |
1.544 |
1.530 |
1.537 |
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| 0 |
2.387 |
2.355 |
2.371 |
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货期
3-5 working days