产品描述
This Pig SDF1a/CXCL12 ELISA Kit was designed for the quantitative measurement of Pig SDF1a/CXCL12 protein in serum, plasma, cell culture supernates, tissue homogenates, cell Lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .
缩写
SDF1a/CXCL12
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig SDF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 89-97 | |
| 1:2 | Average % | 92 | |
| Range % | 88-96 | |
| 1:4 | Average % | 86 | |
| Range % | 82-90 | |
| 1:8 | Average % | 97 | |
| Range % | 92-101 | |
回收率
| The recovery of pig SDF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 98 | 94-102 | |
| EDTA plasma (n=4) | 86 | 82-90 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.812 | 2.701 | 2.757 | 2.564 | | | 10 | 2.397 | 2.361 | 2.379 | 2.186 | | | 5 | 1.798 | 1.712 | 1.755 | 1.562 | | | 2.5 | 1.011 | 0.979 | 0.995 | 0.802 | | | 1.25 | 0.607 | 0.634 | 0.621 | 0.428 | | | 0.625 | 0.403 | 0.427 | 0.415 | 0.222 | | | 0.312 | 0.304 | 0.321 | 0.313 | 0.120 | | | 0 | 0.188 | 0.198 | 0.193 | | |
|
货期
3-5 working days