产品描述
The Rat Transferrin (TF) ELISA Kit is the perfect solution for researchers studying the cardiovascular system. This quantitative assay kit allows for the detection of Serotransferrin (Tf) in serum, plasma, and tissue homogenates of Rattus norvegicus (rat) samples.
With a detection range of 7.8 pmol/mL to 500 pmol/mL and a sensitivity of 1.95 pmol/mL, this ELISA kit provides accurate and reliable results for your research needs. The assay time is quick, ranging from 1-5 hours, and the sample volume required is only 50-100 ul.
The detection wavelength for this kit is 450 nm, providing high sensitivity and specificity for the measurement of Tf in rat samples. The assay principle is based on the sandwich method, providing high specificity and accuracy for the detection of this protein.
别名
Tf ELISA Kit; Serotransferrin ELISA Kit; Transferrin ELISA Kit; Beta-1 metal-binding globulin ELISA Kit; Liver regeneration-related protein LRRG03 ELISA Kit; Siderophilin ELISA Kit
缩写
TF
Uniprot No
P12346
灵敏度
1.95 pmol/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 89 | |
| Range % | 85-92 | |
| 1:200 | Average % | 96 | |
| Range % | 89-104 | |
| 1:400 | Average % | 90 | |
| Range % | 84-96 | |
| 1:800 | Average % | 93 | |
| Range % | 87-99 | |
回收率
| The recovery of rat TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 89-98 | |
| EDTA plasma (n=4) | 94 | 89-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pmol/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.042 | 2.143 | 2.093 | 1.958 | | | 250 | 1.682 | 1.696 | 1.689 | 1.554 | | | 125 | 1.197 | 1.234 | 1.216 | 1.081 | | | 62.5 | 0.803 | 0.819 | 0.811 | 0.676 | | | 31.2 | 0.392 | 0.407 | 0.400 | 0.265 | | | 15.6 | 0.260 | 0.298 | 0.279 | 0.144 | | | 7.8 | 0.173 | 0.180 | 0.177 | 0.042 | | | 0 | 0.134 | 0.136 | 0.135 | | |
|
货期
3-5 working days
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