华美生物/CUSABIORELA小鼠核因子-κB亚基p65亲和肽(NF-κB p65)酶联免疫试剂盒Mouse nuclear factor-κB p65,NF-κB p65 ELISA KitCSB-E08789m华美-易享

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RELA小鼠核因子-κB亚基p65亲和肽(NF-κB p65)酶联免疫试剂盒Mouse nuclear factor-κB p65,NF-κB p65 ELISA Kit

数万篇文献的选择，荣登各大SCI高分期刊

原料试剂研发实验室

价格

￥2500.00-3800.00

品牌华美生物/CUSABIO

地区中国,湖北省,武汉市

货号 CSB-E08789m

产地国产

选择规格

96T

48T

易享客服

武汉华美生物工程有限公司

湖北省武汉东湖新技术开发区神墩五路269号生之源股份生物科创产业园B栋

营业执照已审核

武汉华美生物工程有限公司成立于2007年12月，是一家以科研试剂生产、诊断试剂原料供应为核心，集自主研发、生产、营销为一体的生物高新技术企业。华美生物拥有多项先进的核心技术，致力于为全球高等院校、政府研究机构、诊断试剂生产厂家以及生物制药公司提供高质量产品与高水平的技术服务。华美生物子品牌CUSABIO主要经营科研试剂产品，现拥有各物种基因产品一万余种；现货重组蛋白16,000多种；数十个种属的多克隆抗体65,000多种和千余种高质量单克隆抗体。ELISA试剂盒现已涵盖20多个种属、3,000个靶标、4,000多种产品，涉及肿瘤、激素、自身免疫、心血管、代谢等众多研究领域。华美CUSABIO产品远销欧美日德等九十多个国家和地区，已被众多国际顶级学术期刊Nature, Cell, Immunity, Nature Medicine, Cell Metabolism, Gastroenterology等引用。华美生物总部位于湖北武汉，在美国建立了子公司，并在全球近百个国家拥有授权经销商。华美生物始终秉承“诚信与品质”的核心理念，借助自身的创新实力，匠心打造精品科研试剂与诊断原料，无畏前行，尽情展现着开创者的梦想与未来，为人类健康事业创造价值！

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产品规格图文详情技术文档

产品规格

品牌名称

华美生物/CUSABIO

货号

CSB-E08789m

国产/进口

国产

规格

96T,48T

图文详情

产品描述

The mouse NF-κB subunit p65 ELISA kit is suitable for the quantitative determination of mouse p65 in different sample types, including serum, plasma, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample.

p65, also called RELA, is a member of the NF-κB family of transcriptional regulatory proteins that works as the activating component of the p65/p50 heterodimer. It plays an essential role in cell survival as p65-deficient mice do not survive. Nuclear translocation of the p65/p50 heterodimer is essential for NF-κB signaling. On activation of TNFR1, the TRAF2/5 activates the IKK complex, resulting in IκBα phosphorylation and subsequent ubiquitin-dependent proteasomal degradation. This relieves the NF-κB p65/p50 heterodimer from cytosolic retention, leading to nuclear translocation and target gene activation. Acetylation of p65 affects its biological function. Acetylation on lysine residue 221 is crucial for DNA binding and for inhibiting the interaction between p65 and IκBα.

别名

Rela ELISA Kit; Nfkb3 ELISA Kit; Transcription factor p65 ELISA Kit; Nuclear factor NF-kappa-B p65 subunit ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 ELISA Kit

缩写

RELA

Uniprot No

Q04207

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-100
1:2	Average %	84
	Range %	80-88
1:4	Average %	93
	Range %	89-97
1:8	Average %	89
	Range %	84-93

回收率

The recovery of mouse NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-110
EDTA plasma (n=4)	95	90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.659	2.600	2.630	2.451
1000	2.042	1.935	1.989	1.810
500	1.211	1.270	1.241	1.062
250	0.736	0.724	0.730	0.551
125	0.394	0.408	0.401	0.222
62.5	0.307	0.311	0.309	0.130
31.25	0.258	0.247	0.253	0.074
0	0.175	0.182	0.179

货期

3-5 working days

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