产品描述

The human S100A8 ELISA Kit is engineered for accurate measurement of human S100A8 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the S100A8 content in the sample. The color intensity is positively correlated with S100A8 content in the sample. The S100A8 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

S100A8 binds to cell surface receptors that trigger signaling pathways to participate in the inflammatory process and plays a key role in multiple cellular processes, including cell survival, cell cycle progression, differentiation, proliferation, and cell migration. High expression of S100A8 has been found in various inflammation-related diseases like inflammatory bowel disease and solid cancers like breast cancer. S100A8 contributes to tumor growth, metastasis development, angiogenesis, and immune escape in a wide variety of solid cancers.

别名

60B8Ag ELISA Kit; AI323541 ELISA Kit; B8Ag ELISA Kit; BEE11 ELISA Kit; CAGA ELISA Kit; Calgranulin-A ELISA Kit; Calprotectin L1L subunit ELISA Kit; Calprotectin; included ELISA Kit; CFAG ELISA Kit; CGLA ELISA Kit; Chemotactic cytokine CP-10 ELISA Kit; CP-10 ELISA Kit; Cystic fibrosis antigen ELISA Kit; L1Ag ELISA Kit; Leukocyte L1 complex light chain ELISA Kit; MA387 ELISA Kit; MIF ELISA Kit; Migration inhibitory factor-related protein 8 ELISA Kit; MRP-8 ELISA Kit; Myeloid-related protein 8 ELISA Kit; Neutrophil cytosolic 7 kDa protein ELISA Kit; NIF ELISA Kit; p8 ELISA Kit; Pro-inflammatory S100 cytokine ELISA Kit; Protein S100-A8 ELISA Kit; S100 calcium binding protein A8 (calgranulin A) ELISA Kit; S100 calcium binding protein A8 ELISA Kit; S100 calcium-binding protein A8 ELISA Kit; S100A8 ELISA Kit; S100A8/S100A9 complex; included ELISA Kit; S10A8_HUMAN ELISA Kit; Urinary stone protein band A ELISA Kit

缩写

S100A8

Uniprot No

P05109

灵敏度

0.31 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human S100A8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		83-95
1:2		Average %		95
		Range %		91-99
1:4		Average %		95
		Range %		88-101
1:8		Average %		98
		Range %		91-102

回收率

The recovery of human S100A8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		85		81-93
EDTA plasma (n=4)		95		93-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 80 2.945 2.962 2.954 2.800 40 2.567 2.504 2.536 2.382 20 1.676 1.799 1.738 1.584 10 0.849 0.887 0.868 0.714 5 0.441 0.411 0.426 0.272 2.5 0.263 0.251 0.257 0.103 1.25 0.201 0.211 0.206 0.052 0 0.155 0.152 0.154

货期

3-5 working days