产品描述
This Rat TNF-β ELISA Kit was designed for the quantitative measurement of Rat TNF-β protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.
别名
Lta ELISA Kit; Tnfb ELISA Kit; Tnfsf1 ELISA Kit; Lymphotoxin-alpha ELISA Kit; LT-alpha ELISA Kit; TNF-beta ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit
缩写
TNF-β
Uniprot No
Q06332
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 101 | | |
| Range % | 96-105 | | |
| 1:2 | Average % | 102 | | |
| Range % | 97-107 | | |
| 1:4 | Average % | 87 | | |
| Range % | 83-94 | | |
| 1:8 | Average % | 96 | | |
| Range % | 91-102 | | |
回收率
| The recovery of rat TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 104 | 99108 | | |
| EDTA plasma (n=4) | 88 | 84-93 | | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 500 | 2.632 | 2.765 | 2.699 | 2.614 | | | | 250 | 2.212 | 2.302 | 2.257 | 2.172 | | | | 125 | 1.545 | 1.577 | 1.561 | 1.476 | | | | 62.5 | 0.765 | 0.771 | 0.768 | 0.683 | | | | 31.2 | 0.411 | 0.423 | 0.417 | 0.332 | | | | 15.6 | 0.257 | 0.264 | 0.261 | 0.176 | | | | 7.8 | 0.176 | 0.185 | 0.181 | 0.096 | | | | 0 | 0.084 | 0.085 | 0.085 | | | |
|
货期
3-5 working days