产品描述
This Rat SREBP-1C ELISA Kit was designed for the quantitative measurement of Rat SREBP-1C protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .
别名
Srebf1; Srebp1; Sterol regulatory element-binding protein 1; SREBP-1; Adipocyte determination- and differentiation-dependent factor 1; ADD1; Sterol regulatory element-binding transcription factor 1
缩写
SREBP-1C
Uniprot No
P56720
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SREBP-1C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 90 | |
| Range % | 86-96 | |
| 1:10 | Average % | 101 | |
| Range % | 95-108 | |
| 1:20 | Average % | 95 | |
| Range % | 90-100 | |
| 1:40 | Average % | 92 | |
| Range % | 87-96 | |
回收率
| The recovery of rat SREBP-1C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 90-96 | |
| EDTA plasma (n=4) | 96 | 90-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 4000 | 2.538 | 2.569 | 2.554 | 2.356 | | | 2000 | 1.898 | 1.866 | 1.882 | 1.684 | | | 1000 | 1.381 | 1.351 | 1.366 | 1.168 | | | 500 | 0.959 | 0.999 | 0.979 | 0.781 | | | 250 | 0.574 | 0.587 | 0.581 | 0.383 | | | 125 | 0.364 | 0.381 | 0.373 | 0.175 | | | 62.5 | 0.304 | 0.328 | 0.316 | 0.118 | | | 0 | 0.196 | 0.200 | 0.198 | | |
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货期
3-5 working days