产品描述
The Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit is a powerful research tool designed to detect and quantify Pulmonary surfactant-associated protein B in rat serum, plasma, and tissue homogenates. This kit is specifically designed for use in lung-related disease and signal transduction research and can detect levels of SP-B as low as 0.78 pg/mL with a detection range of 3.12 pg/mL to 200 pg/mL.
The assay principle is based on the sandwich technique, in which the capture antibody is pre-coated on a microplate and the detection antibody is labeled with biotin. Upon binding to the target analyte, the complex is captured by streptavidin-HRP conjugate and the substrate TMB is added to produce a colored reaction product that is quantified by measuring the absorbance at 450 nm.
With an assay time of 1-5 hours, a sample volume of 50-100 µl, and a detection wavelength of 450 nm, the SP-B ELISA kit provides accurate and reliable results for your research needs. With its high sensitivity and wide detection range, it can help you gain a better understanding of the role of SP-B in various physiological and pathological conditions.
别名
Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
缩写
SFTPB
Uniprot No
P22355
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1000 | Average % | 90 | |
| Range % | 85-100 | |
| 1:2000 | Average % | 94 | |
| Range % | 87-98 | |
| 1:4000 | Average % | 90 | |
| Range % | 85-96 | |
| 1:8000 | Average % | 94 | |
| Range % | 90-98 | |
回收率
| The recovery of rat SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 90-101 | |
| EDTA plasma (n=4) | 91 | 85-97 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.470 | 2.589 | 2.530 | 2.377 | | | 100 | 2.115 | 2.124 | 2.120 | 1.967 | | | 50 | 1.713 | 1.726 | 1.720 | 1.567 | | | 25 | 1.315 | 1.356 | 1.336 | 1.183 | | | 12.5 | 0.985 | 0.971 | 0.978 | 0.825 | | | 6.25 | 0.532 | 0.541 | 0.537 | 0.384 | | | 3.12 | 0.378 | 0.389 | 0.384 | 0.231 | | | 0 | 0.152 | 0.154 | 0.153 | | |
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货期
3-5 working days
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