产品描述
CUSABIO's mouse pulmonary surfactant-associated protein C (SP-C) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse SP-C in serum, plasma, cell culture supernates, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of SP-C in samples. The color intensity is measured at 450 nm via a microplate reader.
SP-C (SFTPC) is expressed only in the lung and is a highly specific marker for identifying TII cells. SP-C has several functions in pulmonary surfactant, including increasing the adsorption and spreading of phospholipids at the air-liquid interface thereby promoting the surface tension-lowering properties of surfactant, a role in surfactant recycling and homeostasis, and involvement in the modulation of the innate defense system. Mutations in SP-C protein and its total absence can lead to interstitial lung disease.
别名
Sftpc ELISA Kit; Sftp2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit; SP5 ELISA Kit
缩写
SFTPC
Uniprot No
P21841
灵敏度
0.58 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 80-92 | |
| 1:2 | Average % | 91 | |
| Range % | 85-96 | |
| 1:4 | Average % | 95 | |
| Range % | 90-100 | |
| 1:8 | Average % | 98 | |
| Range % | 92-102 | |
回收率
| The recovery of mouse SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 88-98 | |
| EDTA plasma (n=4) | 96 | 90-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 150 | 2.147 | 2.052 | 2.100 | 2.009 | | | 75 | 1.693 | 1.576 | 1.635 | 1.544 | | | 37.5 | 1.121 | 1.044 | 1.083 | 0.992 | | | 18.8 | 0.648 | 0.639 | 0.644 | 0.553 | | | 9.4 | 0.429 | 0.424 | 0.427 | 0.336 | | | 4.7 | 0.295 | 0.279 | 0.287 | 0.196 | | | 2.3 | 0.194 | 0.179 | 0.187 | 0.096 | | | 0 | 0.092 | 0.090 | 0.091 | | |
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货期
3-5 working days
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