产品描述
This Mouse TIMP3 ELISA Kit was designed for the quantitative measurement of Mouse TIMP3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 46.88 pg/mL-3000 pg/mL and the sensitivity is 11.72 pg/mL.
别名
Timp3 ELISA Kit; Sun ELISA Kit; Timp-3 ELISA Kit; Metalloproteinase inhibitor 3 ELISA Kit; Tissue inhibitor of metalloproteinases 3 ELISA Kit; TIMP-3 ELISA Kit
缩写
TIMP3
Uniprot No
P39876
灵敏度
11.72 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TIMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 100 | | |
| Range % | 95-104 | | |
| 1:2 | Average % | 94 | | |
| Range % | 88-98 | | |
| 1:4 | Average % | 98 | | |
| Range % | 91-102 | | |
| 1:8 | Average % | 97 | | |
| Range % | 90-102 | | |
回收率
| The recovery of mouse TIMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 94 | 87-100 | | |
| EDTA plasma (n=4) | 101 | 95-105 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 3000 | 2.494 | 2.421 | 2.458 | 2.288 | | | | 1500 | 1.893 | 1.856 | 1.875 | 1.705 | | | | 750 | 1.165 | 1.132 | 1.149 | 0.979 | | | | 375 | 0.650 | 0.643 | 0.647 | 0.477 | | | | 187.5 | 0.413 | 0.390 | 0.402 | 0.232 | | | | 93.75 | 0.301 | 0.294 | 0.298 | 0.128 | | | | 46.88 | 0.229 | 0.225 | 0.227 | 0.057 | | | | 0 | 0.169 | 0.171 | 0.170 | | | |
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货期
3-5 working days