产品描述

This Human SERPIND1 ELISA Kit was designed for the quantitative measurement of Human SERPIND1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 pg/mL-80 pg/mL and the sensitivity is 0.312 pg/mL.

别名

D22S673 ELISA Kit; HC II ELISA Kit; HC-II ELISA Kit; HC2 ELISA Kit; HCF2 ELISA Kit; HEP2_HUMAN ELISA Kit; Heparin cofactor 2 ELISA Kit; Heparin cofactor II ELISA Kit; HLS2 ELISA Kit; Leuserpin 2 ELISA Kit; LS2 ELISA Kit; Protease inhibitor leuserpin 2 ELISA Kit; Protease inhibitor leuserpin-2 ELISA Kit; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1 ELISA Kit; Serpin D1 ELISA Kit; serpin peptidase inhibitor, clade D (heparin cofactor), member 1 ELISA Kit; SERPIND1 ELISA Kit; THPH10 ELISA Kit

缩写

SERPIND1

Uniprot No

P05546

灵敏度

0.312 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human HCⅡ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		92
		Range %		86-98
1:200		Average %		95
		Range %		91-100
1:400		Average %		98
		Range %		90-105
1:800		Average %		95
		Range %		89-100

回收率

The recovery of human HCⅡspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		92-102
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 80 2.718 2.785 2.752 2.668 40 2.239 2.143 2.191 2.107 20 1.361 1.387 1.374 1.290 10 0.758 0.742 0.750 0.666 5 0.452 0.418 0.435 0.351 2.5 0.227 0.219 0.223 0.139 1.25 0.184 0.188 0.186 0.102 0 0.085 0.083 0.084

货期

3-5 working days