产品描述

CUSABIO's human proteinase 3 (PR3) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PR3 in serum, tissue homogenates, plasma, or urine. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of PR3 in samples. The color intensity is measured at 450 nm via a microplate reader.

PR3 (PRTN3) is an enzyme released during neutrophilic inflammation and is capable of cleaving many targets including key structural proteins of the lung. In addition to cleaving structural proteins resulting in tissue remodeling, PR3 also assists in the defensive immune role of the neutrophil including modulating various cellular activities, cleaving host protein into antibacterial peptides, and activating pro-inflammatory cytokines. PR3 also regulates a variety of cytokine functions, which impact processes such as metabolism and inflammasome generation. It also exerts a pro-inflammatory role by interacting with the complement pathway.

别名

ACPA ELISA Kit; AGP 7 ELISA Kit; AGP7 ELISA Kit; AGP7 serine proteinase ELISA Kit; Azurophil Granule Protein 7 ELISA Kit; C ANCA ELISA Kit; C ANCA antigen ELISA Kit; C-ANCA antigen ELISA Kit; CANCA ELISA Kit; EC 3.4.21.76 ELISA Kit; Leukocyte proteinase 3 ELISA Kit; MBN ELISA Kit; MBT ELISA Kit; MBT WEGENER AUTOANTIGEN ELISA Kit; Myeloblastin ELISA Kit; Neutrophil proteinase 4 ELISA Kit; NP 4 ELISA Kit; NP-4 ELISA Kit; NP4 ELISA Kit; P29 ELISA Kit; PR 3 ELISA Kit; PR-3 ELISA Kit; PR3 ELISA Kit; Proteinase 3 ELISA Kit; Proteinase3 ELISA Kit; PRTN 3 ELISA Kit; Prtn3 ELISA Kit; PRTN3_HUMAN ELISA Kit; Serine proteinase neutrophil Wegener granulomatosis autoantigen ELISA Kit; Serine proteinase; neutrophil ELISA Kit; Wegener autoantigen ELISA Kit; Wegener granulomatosis autoantigen ELISA Kit

缩写

PRTN3

Uniprot No

P24158

灵敏度

0.195 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PR3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		86-95
1:2		Average %		88
		Range %		81-93
1:4		Average %		98
		Range %		93-102
1:8		Average %		102
		Range %		98-106

回收率

The recovery of human PR3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		104		94-110
EDTA plasma (n=4)		92		88-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 50 2.002 2.050 2.026 1.934 25 1.503 1.584 1.544 1.452 12.5 1.014 1.101 1.058 0.966 6.25 0.598 0.606 0.602 0.510 3.12 0.384 0.387 0.386 0.294 1.56 0.295 0.301 0.298 0.206 0.78 0.197 0.189 0.193 0.101 0 0.095 0.089 0.092

货期

3-5 working days