产品描述
This Rabbit TCC C5b-9 ELISA Kit was designed for the quantitative measurement of Rabbit TCC C5b-9 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL .
缩写
TCC C5b-9
灵敏度
1.95 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 80-92 | |
| 1:2 | Average % | 96 | |
| Range % | 91-105 | |
| 1:4 | Average % | 97 | |
| Range % | 92-103 | |
| 1:8 | Average % | 91 | |
| Range % | 86-98 | |
回收率
| The recovery of rabbit TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 89-109 | |
| EDTA plasma (n=4) | 97 | 92-108 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.659 | 2.563 | 2.611 | 2.519 | | | 250 | 2.240 | 2.128 | 2.184 | 2.092 | | | 125 | 1.488 | 1.375 | 1.432 | 1.340 | | | 62.5 | 0.845 | 0.817 | 0.831 | 0.739 | | | 31.2 | 0.444 | 0.434 | 0.439 | 0.347 | | | 15.6 | 0.247 | 0.239 | 0.243 | 0.151 | | | 7.8 | 0.156 | 0.148 | 0.152 | 0.060 | | | 0 | 0.093 | 0.091 | 0.092 | | |
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货期
3-5 working days