产品描述
SOD(超氧化物歧化酶,superoxide dismutase)是一种抗氧化酶,参与细胞清除有害自由基,维护细胞内外环境的稳定。
华美生物所提供的Fish Superoxide Dismutase(SOD)ELISA kit属于ELISA检测试剂盒,采用竞争法定量检测鱼类血清、血浆、组织匀浆样本中的SOD,其灵敏度为0.625 ng/mL,检测范围为1.25 ng/mL-20 ng/mL。
别名
sod1 ELISA Kit; cuzn ELISA Kit; Superoxide dismutase [Cu-Zn] ELISA Kit; EC 1.15.1.1 ELISA Kit
缩写
SOD
Uniprot No
O73872
灵敏度
0.625 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of fish SOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:100 |
Average % |
95 |
|
| Range % |
92-98 |
|
| 1:200 |
Average % |
103 |
|
| Range % |
100-106 |
|
| 1:400 |
Average % |
98 |
|
| Range % |
94-102 |
|
| 1:800 |
Average % |
92 |
|
| Range % |
88-96 |
|
回收率
| The recovery of fish SOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
99 |
96-103 |
|
| EDTA plasma (n=4) |
89 |
85-93 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
|
|
| 20 |
0.092 |
0.099 |
0.096 |
|
|
| 10 |
0.143 |
0.152 |
0.148 |
|
|
| 5 |
0.245 |
0.223 |
0.234 |
|
|
| 2.5 |
0.588 |
0.575 |
0.582 |
|
|
| 1.25 |
1.397 |
1.402 |
1.400 |
|
|
| 0 |
2.214 |
2.352 |
2.283 |
|
|
|
货期
3-5 working days