产品描述
TFPI,全称为Tissue Factor Pathway Inhibitor,是一种天然的抗凝血蛋白质。TFPI主要通过抑制凝血酶在TF-FVIIa复合物上的活性,从而抑制凝血级联反应的进行。此外,TFPI还能够抑制细胞外基质的降解、细胞增殖和炎症反应等生物学过程。在正常情况下,TFPI的含量比较低,通常在几ng/mL的水平范围内。
华美生物所提供的Human Tissue factor pathway inhibitor,TFPI ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的TFPI,其灵敏度为0.293 ng/mL,检测范围为0.782 ng/mL-50 ng/mL。
别名
Anti convertin ELISA Kit; EPI ELISA Kit; Extrinsic pathway inhibitor ELISA Kit; LACI ELISA Kit; Lipoprotein associated coagulation inhibitor ELISA Kit; Lipoprotein-associated coagulation inhibitor ELISA Kit; TFI ELISA Kit; TFPI 1 ELISA Kit; TFPI ELISA Kit; TFPI1 ELISA Kit; TFPI1_HUMAN ELISA Kit; Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor) ELISA Kit; Tissue factor pathway inhibitor ELISA Kit
缩写
TFPI
Uniprot No
P10646
灵敏度
0.293 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human TFPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
93 |
|
| Range % |
88-97 |
|
| 1:2 |
Average % |
101 |
|
| Range % |
97-105 |
|
| 1:4 |
Average % |
94 |
|
| Range % |
89-98 |
|
| 1:8 |
Average % |
96 |
|
| Range % |
91-102 |
|
回收率
| The recovery of human TFPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
89-98 |
|
| EDTA plasma (n=4) |
86 |
81-92 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 50 |
2.519 |
2.623 |
2.571 |
2.440 |
|
| 25 |
2.021 |
2.154 |
2.088 |
1.957 |
|
| 12.5 |
1.434 |
1.456 |
1.445 |
1.314 |
|
| 6.25 |
0.813 |
0.838 |
0.826 |
0.695 |
|
| 3.125 |
0.436 |
0.445 |
0.441 |
0.310 |
|
| 1.563 |
0.304 |
0.310 |
0.307 |
0.176 |
|
| 0.782 |
0.221 |
0.216 |
0.219 |
0.088 |
|
| 0 |
0.130 |
0.131 |
0.131 |
|
|
|
货期
3-5 working days