产品描述

The human SIRT1 ELISA Kit is intended to measure human SIRT1 in serum, plasma, cell culture supernates,, or tissue homogenates. This assay uses the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human SIRT1 concentration. The intensity of the colored product is directly proportional to the concentration of SIRT1 present in the sample. This human SIRT1 ELISA kit has high specificity and excellent sensitivity.

SIRT1 is a conserved class III histone deacetylase that is mainly located in the nucleus and deacetylates histones to influence the state of the chromatin and subsequent gene transcription in rapid response to cellular conditions of energy demand. It is involved in a plethora of physiological activities, including controlling DNA repair, tissue regeneration, cell survival, proliferation, inflammation, neuronal signaling, aging, and circadian rhythms. It also modulates especially energy metabolism and stress responses. In alcoholic liver diseases (ALDs), SIRT1 is beneficial for the regulation of hepatic lipid metabolism, inhibition of hepatic inflammation, controlling of hepatic fibrosis, and mediation of hepatocellular carcinoma.

别名

75SirT1 ELISA Kit; hSIR2 ELISA Kit; hSIRT1 ELISA Kit; HST2 ELISA Kit; HST2, S. cerevisiae, homolog of ELISA Kit; NAD dependent deacetylase sirtuin 1 ELISA Kit; NAD dependent protein deacetylase sirtuin 1 ELISA Kit; NAD-dependent deacetylase sirtuin-1 ELISA Kit; OTTHUMP00000198111 ELISA Kit; OTTHUMP00000198112 ELISA Kit; Regulatory protein SIR2 homolog 1 ELISA Kit; SIR1_HUMAN ELISA Kit; SIR2 ELISA Kit; SIR2 like 1 ELISA Kit; SIR2 like protein 1 ELISA Kit; SIR2, S.cerevisiae, homolog-like 1 ELISA Kit; SIR2-like protein 1 ELISA Kit; SIR2ALPHA ELISA Kit; SIR2L1 ELISA Kit; Sirt1 ELISA Kit; SirtT1 75 kDa fragment ELISA Kit; Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) ELISA Kit; Sirtuin 1 ELISA Kit; Sirtuin type 1 ELISA Kit

缩写

SIRT1

Uniprot No

Q96EB6

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SIRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		89-94
1:2		Average %		100
		Range %		97-102
1:4		Average %		93
		Range %		87-96
1:8		Average %		99
		Range %		94-102

回收率

The recovery of human SIRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		90-97
EDTA plasma (n=4)		96		93-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.322 2.335 2.329 2.187 5 1.776 1.850 1.813 1.671 2.5 1.278 1.298 1.288 1.146 1.25 0.929 0.918 0.924 0.782 0.625 0.640 0.623 0.632 0.490 0.312 0.449 0.440 0.445 0.303 0.156 0.303 0.288 0.296 0.154 0 0.144 0.140 0.142

货期

3-5 working days