产品描述

This Human PRSS3 ELISA Kit was designed for the quantitative measurement of Human PRSS3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

Brain trypsinogen ELISA Kit; Mesotrypsin ELISA Kit; Mesotrypsinogen ELISA Kit; MTG ELISA Kit; Pancreatic trypsinogen III ELISA Kit; Protease, serine, 3 ELISA Kit; Protease, serine, 4 (trypsin 4, brain) ELISA Kit; PRSS3 ELISA Kit; PRSS4 ELISA Kit; Serine protease 3 ELISA Kit; Serine protease 4 ELISA Kit; T9 ELISA Kit; TRY3 ELISA Kit; TRY3_HUMAN ELISA Kit; TRY4 ELISA Kit; Trypsin 3 ELISA Kit; Trypsin III ELISA Kit; Trypsin IV ELISA Kit; Trypsin-3 ELISA Kit; Trypsinogen 4 ELISA Kit; Trypsinogen 5 ELISA Kit; Trypsinogen IV ELISA Kit

缩写

PRSS3

Uniprot No

P35030

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PRSS3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		92
		Range %		87-96
1:200		Average %		103
		Range %		98-107
1:400		Average %		105
		Range %		101-109
1:800		Average %		92
		Range %		86-98

回收率

The recovery of human PRSS3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		86-98
EDTA plasma (n=4)		97		91-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1000 2.584 2.687 2.636 2.552 500 2.295 2.165 2.230 2.146 250 1.594 1.523 1.559 1.475 125 0.889 0.876 0.883 0.799 62.5 0.441 0.454 0.448 0.364 31.2 0.261 0.252 0.257 0.173 15.6 0.143 0.148 0.146 0.062 0 0.083 0.084 0.084

货期

3-5 working days