产品描述
This Goat TNF ELISA Kit was designed for the quantitative measurement of Goat TNF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
缩写
TNF
Uniprot No
P13296
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of goat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 84 | |
| Range % | 81-91 | |
| 1:2 | Average % | 98 | |
| Range % | 91-103 | |
| 1:4 | Average % | 99 | |
| Range % | 95-104 | |
| 1:8 | Average % | 94 | |
| Range % | 86-98 | |
回收率
| The recovery of goat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 90-97 | |
| EDTA plasma (n=4) | 97 | 92-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.601 | 2.589 | 2.595 | 2.493 | | | 500 | 2.154 | 2.133 | 2.144 | 2.042 | | | 250 | 1.658 | 1.621 | 1.640 | 1.538 | | | 125 | 1.123 | 1.114 | 1.119 | 1.017 | | | 62.5 | 0.712 | 0.665 | 0.689 | 0.587 | | | 31.2 | 0.413 | 0.399 | 0.406 | 0.304 | | | 15.6 | 0.256 | 0.242 | 0.249 | 0.147 | | | 0 | 0.104 | 0.100 | 0.102 | | |
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货期
3-5 working days