产品描述
This Human TLR9 ELISA Kit was designed for the quantitative measurement of Human TLR9 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.
别名
CD 289 ELISA Kit; CD289 ELISA Kit; TLR 9 ELISA Kit; TLR9 ELISA Kit; TLR9_HUMAN ELISA Kit; Toll like receptor 9 ELISA Kit; Toll like receptor 9 isoform A precursor ELISA Kit; Toll like receptor 9 isoform B ELISA Kit; Toll-like receptor 9 ELISA Kit
缩写
TLR9
Uniprot No
Q9NR96
灵敏度
19.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human TLR-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 92-104 | |
| 1:2 | Average % | 84 | |
| Range % | 80-88 | |
| 1:4 | Average % | 90 | |
| Range % | 85-94 | |
| 1:8 | Average % | 93 | |
| Range % | 89-97 | |
回收率
| The recovery of human TLR-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 105 | 100-112 | |
| EDTA plasma (n=4) | 94 | 90-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | 5000 | 2.627 | 2.517 | 2.572 | 2.472 | | | 2500 | 1.943 | 2.026 | 1.985 | 1.885 | | | 1250 | 1.339 | 1.302 | 1.321 | 1.221 | | | 625 | 0.714 | 0.775 | 0.745 | 0.645 | | | 312.5 | 0.405 | 0.384 | 0.395 | 0.295 | | | 156.25 | 0.261 | 0.273 | 0.267 | 0.167 | | | 78 | 0.177 | 0.187 | 0.182 | 0.082 | | | 0 | 0.102 | 0.098 | 0.100 | | |
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货期
3-5 working days