产品描述

This Human REG1A ELISA Kit was designed for the quantitative measurement of Human REG1A protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

ICRF ELISA Kit; Islet cells regeneration factor ELISA Kit; Islet of Langerhans regenerating protein ELISA Kit; Lithostathine 1 alpha ELISA Kit; Lithostathine 1 alpha precursor ELISA Kit; Lithostathine ELISA Kit; Lithostathine-1-alpha ELISA Kit; MGC12447 ELISA Kit; P19 ELISA Kit; Pancreatic stone protein ELISA Kit; Pancreatic stone protein secretory ELISA Kit; Pancreatic thread protein ELISA Kit; Protein X ELISA Kit; PSP ELISA Kit; PSPS ELISA Kit; PSPS1 ELISA Kit; PTP ELISA Kit; RATRGPI ELISA Kit; REG ELISA Kit; REG-1-alpha ELISA Kit; Reg1 ELISA Kit; REG1A ELISA Kit; REG1A_HUMAN ELISA Kit; Regenerating islet derived 1 alpha ELISA Kit; Regenerating islet derived 1 alpha pancreatic stone protein pancreatic thread protein ELISA Kit; Regenerating islet-derived protein 1-alpha ELISA Kit; Regenerating protein I alpha ELISA Kit

缩写

REG1A

Uniprot No

P05451

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human REG1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		87
		Range %		82-93
1:2		Average %		85
		Range %		81-89
1:4		Average %		93
		Range %		83-97
1:8		Average %		95
		Range %		91-99

回收率

The recovery of human REG1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		94-102
EDTA plasma (n=4)		102		96-108

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.608 2.514 2.561 2.426 5 1.893 1.787 1.840 1.705 2.5 1.147 1.085 1.116 0.981 1.25 0.627 0.607 0.617 0.482 0.625 0.374 0.367 0.371 0.236 0.312 0.287 0.293 0.290 0.155 0.156 0.231 0.226 0.229 0.094 0 0.136 0.134 0.135

货期

3-5 working days