别名

AA986889 ELISA Kit; NMD ELISA Kit; OTTHUMP00000215248 ELISA Kit; OTTHUMP00000215249 ELISA Kit; OTTHUMP00000215250 ELISA Kit; Phosphatidylserine specific phospholipase A1 ELISA Kit; Phosphatidylserine specific phospholipase A1alpha ELISA Kit; Phosphatidylserine-specific phospholipase A1 ELISA Kit; Phospholipase A1 member A ELISA Kit; pla1a ELISA Kit; PLA1A_HUMAN ELISA Kit; PS PLA1 ELISA Kit; PS-PLA1 ELISA Kit; PSPLA 1 ELISA Kit; PSPLA1 ELISA Kit

缩写

PLA1A

Uniprot No

Q53H76

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PLA1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		82-95
1:2		Average %		97
		Range %		93-101
1:4		Average %		99
		Range %		95-106
1:8		Average %		93
		Range %		87-99

回收率

The recovery of human PLA1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		87-95
EDTA plasma (n=4)		99		95-104

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected     20 2.340 2.344 2.342 2.237     10 1.604 1.741 1.673 1.568     5 0.936 0.968 0.952 0.847     2.5 0.576 0.554 0.565 0.460     1.25 0.353 0.325 0.339 0.234     0.625 0.230 0.213 0.222 0.117     0.312 0.185 0.180 0.183 0.078     0 0.104 0.106 0.105

货期

3-5 working days