产品描述

This Human PPARG ELISA Kit was designed for the quantitative measurement of Human PPARG protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

别名

CIMT1 ELISA Kit; GLM1 ELISA Kit; NR1C3 ELISA Kit; Nuclear receptor subfamily 1 group C member 3 ELISA Kit; OTTHUMP00000185032 ELISA Kit; OTTHUMP00000185036 ELISA Kit; Peroxisome proliferator activated nuclear receptor gamma variant 1 ELISA Kit; Peroxisome proliferator activated receptor gamma 1 ELISA Kit; Peroxisome Proliferator Activated Receptor gamma ELISA Kit; Peroxisome proliferator-activated receptor gamma ELISA Kit; PPAR gamma ELISA Kit; PPAR-gamma ELISA Kit; PPARG ELISA Kit; PPARG_HUMAN ELISA Kit; PPARG1 ELISA Kit; PPARG2 ELISA Kit; PPARgamma ELISA Kit

缩写

PPARG

Uniprot No

P37231

灵敏度

19.5 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PPAR-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		86-99
1:2		Average %		94
		Range %		84-99
1:4		Average %		90
		Range %		85-96
1:8		Average %		86
		Range %		80-94

回收率

The recovery of human PPAR-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		89-102
EDTA plasma (n=4)		89		84-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 5000 2.315 2.215 2.265 2.119 2500 1.817 1.717 1.767 1.621 1250 1.257 1.157 1.207 1.061 625 0.712 0.702 0.707 0.561 312 0.418 0.408 0.413 0.267 156 0.331 0.321 0.326 0.180 78 0.255 0.245 0.250 0.104 0 0.151 0.141 0.146

货期

3-5 working days