产品描述

This Human SIRPA ELISA Kit was designed for the quantitative measurement of Human SIRPA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

Signal regulatory protein alpha type 1 ELISA Kit; Bit ELISA Kit; Brain Ig like molecule with tyrosine based activation motifs ELISA Kit; Brain Ig-like molecule with tyrosine-based activation motifs ELISA Kit; Brain immunoglobulin like molecule with tyrosine based activation motifs ELISA Kit; CD172 antigen like family member A ELISA Kit; CD172 antigen-like family member A ELISA Kit; CD172a ELISA Kit; CD172a antigen ELISA Kit; Inhibitory receptor SHPS-1 ELISA Kit; Macrophage fusion receptor ELISA Kit; MFR ELISA Kit; MYD 1 ELISA Kit; Myd 1 antigen ELISA Kit; MyD-1 antigen ELISA Kit; p84 ELISA Kit; Protein tyrosine phosphatase non receptor type substrate 1 ELISA Kit; PTPNS1 ELISA Kit; SHP substrate 1 ELISA Kit; SHPS-1 ELISA Kit; SHPS1 ELISA Kit; SHPS1_HUMAN ELISA Kit; Signal regulatory protein alpha 2 ELISA Kit; Signal regulatory protein alpha 3 ELISA Kit; Signal regulatory protein alpha ELISA Kit; Signal regulatory protein alpha type 2 ELISA Kit; Signal-regulatory protein alpha-1 ELISA Kit; Signal-regulatory protein alpha-2 ELISA Kit; Signal-regulatory protein alpha-3 ELISA Kit; SIRP ELISA Kit; Sirp-alpha-1 ELISA Kit; Sirp-alpha-2 ELISA Kit; Sirp-alpha-3 ELISA Kit; SIRPA ELISA Kit; SIRPalpha ELISA Kit; SIRPalpha1 ELISA Kit; SIRPalpha2 ELISA Kit; SIRPalpha3 ELISA Kit; Tyrosine phosphatase SHP substrate 1 ELISA Kit; Tyrosine protein phosphatase non receptor type substrate 1 ELISA Kit; Tyrosine-protein phosphatase non-receptor type substrate 1 ELISA Kit

缩写

SIRPA

Uniprot No

P78324

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SIRPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		85-96
1:2		Average %		96
		Range %		91-105
1:4		Average %		98
		Range %		95-106
1:8		Average %		86
		Range %		80-92

回收率

The recovery of human SIRPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		86-96
EDTA plasma (n=4)		92		88-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.228 2.128 2.178 2.064 5 1.527 1.458 1.493 1.379 2.5 0.868 0.838 0.853 0.739 1.25 0.427 0.417 0.422 0.308 0.625 0.298 0.288 0.293 0.179 0.312 0.220 0.217 0.219 0.105 0.156 0.174 0.170 0.172 0.058 0 0.115 0.112 0.114

货期

3-5 working days