产品描述
This Mouse PLAT ELISA Kit was designed for the quantitative measurement of Mouse PLAT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.
别名
Plat ELISA Kit; Tissue-type plasminogen activator ELISA Kit; t-PA ELISA Kit; t-plasminogen activator ELISA Kit; tPA ELISA Kit; EC 3.4.21.68) [Cleaved into: Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B] ELISA Kit
缩写
PLAT
Uniprot No
P11214
灵敏度
31.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse t-PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 96 | | |
| Range % | 93-99 | | |
| 1:2 | Average % | 104 | | |
| Range % | 100-108 | | |
| 1:4 | Average % | 86 | | |
| Range % | 83-90 | | |
| 1:8 | Average % | 104 | | |
| Range % | 100-108 | | |
回收率
| The recovery of mouse t-PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 89 | 85-93 | | |
| EDTA plasma (n=4) | 102 | 98-106 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 8000 | 2.076 | 2.000 | 2.038 | 1.865 | | | | 4000 | 1.453 | 1.501 | 1.477 | 1.304 | | | | 2000 | 0.947 | 0.938 | 0.943 | 0.770 | | | | 1000 | 0.564 | 0.559 | 0.562 | 0.389 | | | | 500 | 0.348 | 0.351 | 0.350 | 0.177 | | | | 250 | 0.241 | 0.246 | 0.244 | 0.071 | | | | 125 | 0.205 | 0.200 | 0.203 | 0.030 | | | | 0 | 0.172 | 0.174 | 0.173 | | | |
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货期
3-5 working days