产品描述
This Bovine SFTPC ELISA Kit was designed for the quantitative measurement of Bovine SFTPC protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.
别名
SFTPC ELISA Kit; SFTP2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit
缩写
SFTPC
Uniprot No
P15783
灵敏度
3.12 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 93-99 | |
| 1:2 | Average % | 110 | |
| Range % | 107-113 | |
| 1:4 | Average % | 95 | |
| Range % | 91-99 | |
| 1:8 | Average % | 90 | |
| Range % | 87-93 | |
回收率
| The recovery of bovine SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 87-96 | |
| EDTA plasma (n=4) | 106 | 101-110 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 800 | 2.197 | 2.168 | 2.183 | 2.028 | | | 400 | 1.705 | 1.732 | 1.719 | 1.564 | | | 200 | 1.192 | 1.168 | 1.180 | 1.025 | | | 100 | 0.658 | 0.689 | 0.674 | 0.519 | | | 50 | 0.451 | 0.468 | 0.460 | 0.305 | | | 25 | 0.367 | 0.368 | 0.368 | 0.213 | | | 12.5 | 0.268 | 0.251 | 0.260 | 0.105 | | | 0 | 0.154 | 0.156 | 0.155 | | |
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货期
3-5 working days