产品描述

This Human SERPINB1 ELISA Kit was designed for the quantitative measurement of Human SERPINB1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

别名

Anti elastase ELISA Kit; EI ELISA Kit; ELANH2 ELISA Kit; Epididymis luminal protein 57 ELISA Kit; HEL57 ELISA Kit; ILEU_HUMAN ELISA Kit; LEI ELISA Kit; Leukocyte elastase inhibitor ELISA Kit; M/NEI ELISA Kit; MNEI ELISA Kit; Monocyte/neutrophil elastase inhibitor ELISA Kit; Peptidase inhibitor 2 ELISA Kit; Pi-2 ELISA Kit; PI2 ELISA Kit; Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 1 ELISA Kit; Serpin B1 ELISA Kit; Serpin peptidase inhibitor clade B (ovalbumin) member 1 ELISA Kit; serpinb1 ELISA Kit

缩写

SERPINB1

Uniprot No

P30740

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		97
		Range %		88-101
1:2		Average %		93
		Range %		84-97
1:4		Average %		91
		Range %		85-95
1:8		Average %		98
		Range %		90-101

回收率

The recovery of human SERPINB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		102		94-107
EDTA plasma (n=4)		98		90-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 40 1.768 1.700 1.734 1.606 20 1.409 1.434 1.422 1.294 10 0.886 0.944 0.915 0.787 5 0.565 0.551 0.558 0.430 2.5 0.379 0.363 0.371 0.243 1.25 0.245 0.252 0.249 0.121 0.625 0.201 0.197 0.199 0.071 0 0.130 0.126 0.128

货期

3-5 working days