产品描述
SERPINA4(Kallistatin)是一种由肝脏合成的血浆蛋白,是卡利肽的主要内源性抑制剂。卡利肽是一种能够激活一系列内源性和外源性的蛋白酶的降压肽,对于调节血管紧张素和一氧化氮(NO)等物质的代谢和释放具有重要作用。
华美生物所提供的Human Kallistatin(SERPINA4) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的SERPINA4,其灵敏度为0.039 ng/mL,检测范围为0.156 ng/mL-10 ng/mL。
别名
KAIN_HUMAN ELISA Kit; KAL ELISA Kit; Kallikrein binding protein ELISA Kit; Kallikrein inhibitor ELISA Kit; Kallistatin ELISA Kit; KBP ELISA Kit; KLST ELISA Kit; KST ELISA Kit; MGC108582 ELISA Kit; Peptidase inhibitor 4 ELISA Kit; PI-4 ELISA Kit; PI4 ELISA Kit; Protease inhibitor 4 (kallistatin) ELISA Kit; Protease inhibitor 4 ELISA Kit; Serine (or cysteine) proteinase inhibitor; clade A (alpha 1 antiproteinase; antitrypsin); member 4 ELISA Kit; Serpin A4 ELISA Kit; Serpin peptidase inhibitor clade A member 4 ELISA Kit; Serpin peptidase inhibitor; clade A (alpha 1 antiproteinase; antitrypsin); member 4 ELISA Kit; SERPINA4 ELISA Kit; Tissue kallistatin inhibitor ELISA Kit
缩写
SERPINA4
Uniprot No
P29622
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5000 |
Average % |
92 |
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| Range % |
87-98 |
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| 1:10000 |
Average % |
103 |
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| Range % |
98-108 |
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| 1:20000 |
Average % |
92 |
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| Range % |
90-98 |
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| 1:40000 |
Average % |
94 |
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| Range % |
92-96 |
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回收率
| The recovery of human SERPINA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
93-104 |
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| EDTA plasma (n=4) |
94 |
89-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.432 |
2.565 |
2.499 |
2.337 |
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| 5 |
2.056 |
2.066 |
2.061 |
1.899 |
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| 2.5 |
1.442 |
1.457 |
1.450 |
1.288 |
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| 1.25 |
0.889 |
0.956 |
0.923 |
0.761 |
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| 0.625 |
0.535 |
0.544 |
0.540 |
0.378 |
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| 0.312 |
0.355 |
0.375 |
0.365 |
0.203 |
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| 0.156 |
0.268 |
0.274 |
0.271 |
0.109 |
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| 0 |
0.168 |
0.155 |
0.162 |
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货期
3-5 working days