产品描述
This Rat TP53 ELISA Kit was designed for the quantitative measurement of Rat TP53 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.
缩写
TP53
Uniprot No
P10361
灵敏度
3.12 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat p53/TP53 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 100 | |
| Range % | 95-108 | |
| 1:2 | Average % | 87 | |
| Range % | 82-91 | |
| 1:4 | Average % | 90 | |
| Range % | 85-94 | |
| 1:8 | Average % | 92 | |
| Range % | 88-96 | |
回收率
| The recovery of rat p53/TP53 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 84-93 | |
| EDTA plasma (n=4) | 95 | 90-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 800 | 2.479 | 2.615 | 2.547 | 2.405 | | | 400 | 2.009 | 1.974 | 1.992 | 1.850 | | | 200 | 1.356 | 1.330 | 1.343 | 1.201 | | | 100 | 0.874 | 0.929 | 0.902 | 0.760 | | | 50 | 0.532 | 0.571 | 0.552 | 0.410 | | | 25 | 0.401 | 0.425 | 0.413 | 0.271 | | | 12.5 | 0.297 | 0.287 | 0.292 | 0.150 | | | 0 | 0.140 | 0.143 | 0.142 | | |
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货期
3-5 working days