产品描述
This Rabbit TNNI3 ELISA Kit was designed for the quantitative measurement of Rabbit TNNI3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.
别名
TNNI3 ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
缩写
TNNI3
Uniprot No
P02646
灵敏度
11.75 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 81-92 | |
| 1:2 | Average % | 96 | |
| Range % | 92-99 | |
| 1:4 | Average % | 94 | |
| Range % | 89-98 | |
| 1:8 | Average % | 92 | |
| Range % | 88-96 | |
回收率
| The recovery of rabbit cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 87 | 83-94 | |
| EDTA plasma (n=4) | 89 | 85-97 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 3000 | 2.754 | 2.559 | 2.657 | 2.564 | | | 1500 | 2.115 | 2.020 | 2.068 | 1.975 | | | 750 | 1.248 | 1.227 | 1.238 | 1.145 | | | 375 | 0.658 | 0.677 | 0.668 | 0.575 | | | 187.5 | 0.403 | 0.412 | 0.408 | 0.315 | | | 94 | 0.264 | 0.278 | 0.271 | 0.178 | | | 47 | 0.151 | 0.147 | 0.149 | 0.056 | | | 0 | 0.093 | 0.092 | 0.093 | | |
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货期
3-5 working days