产品描述

This human paraoxonase (PON1) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human PON1 in different samples, including serum, plasma, cell culture supernates, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of PON1 bound in the initial step.

PON1 is a phase-I enzyme involved in the hydrolysis of organophosphate esters. As a high-density lipoprotein (HDL)-associated esterase, PON1 can hydrolyze oxidized LDL-cholesterol, with potential atheroprotective effects. IT also mediates protection against lipid oxidation. PON1 not only has a weak paraoxonase activity but also exhibits anti-oxidant and anti-inflammatory activities. It is implicated in several human diseases, including diabetes mellitus and atherosclerosis. Low PON1 activity has been related to the elevated risk of major cardiovascular events.

别名

A esterase 1 ELISA Kit; A-esterase 1 ELISA Kit; Aromatic esterase 1 ELISA Kit; Arylesterase 1 ELISA Kit; Arylesterase B type ELISA Kit; ESA ELISA Kit; Esterase A ELISA Kit; K 45 ELISA Kit; K-45 ELISA Kit; MVCD5 ELISA Kit; Paraoxonase 1 ELISA Kit; Paraoxonase ELISA Kit; Paraoxonase B type ELISA Kit; Paraoxonase, plasma ELISA Kit; Paraoxonase1 ELISA Kit; PON 1 ELISA Kit; PON ELISA Kit; PON1 ELISA Kit; PON1_HUMAN ELISA Kit; Serum aryldiakylphosphatase ELISA Kit; Serum aryldialkylphosphatase 1 ELISA Kit; Serum paraoxonase/arylesterase 1 ELISA Kit

缩写

PON1

Uniprot No

P27169

灵敏度

7.81 mIU/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PON in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		88
		Range %		80-92
1:200		Average %		98
		Range %		91-105
1:400		Average %		103
		Range %		92-110
1:800		Average %		93
		Range %		86-98

回收率

The recovery of human PON spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		89-98
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml OD1 OD2 Average Corrected 2000 2.655 2.603 2.629 2.521 1000 2.292 2.248 2.283 2.175 500 1.689 1.663 1.676 1.568 250 1.025 0.989 1.007 0.899 125 0.597 0.603 0.600 0.492 62.5 0.409 0.398 0.404 0.296 31.25 0.218 0.229 0.224 0.116 0 0.109 0.107 0.108

货期

3-5 working days