产品描述

This Human TRPV4 ELISA Kit was designed for the quantitative measurement of Human TRPV4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

BCYM3 ELISA Kit; CMT2C ELISA Kit; HMSN2C ELISA Kit; osm 9 like TRP channel 4 ELISA Kit; Osm-9-like TRP channel 4 ELISA Kit; OSM9 like transient receptor potential channel 4 ELISA Kit; Osmosensitive transient receptor potential channel 4 ELISA Kit; OTRPC 4 ELISA Kit; OTRPC4 ELISA Kit; SMAL ELISA Kit; SPSMA ELISA Kit; SSQTL1 ELISA Kit; Transient receptor potential cation channel subfamily V member 4 ELISA Kit; Transient receptor potential protein 12 ELISA Kit; TRP 12 ELISA Kit; TRP12 ELISA Kit; TRPV 4 ELISA Kit; TrpV4 ELISA Kit; TRPV4_HUMAN ELISA Kit; Vanilloid receptor like channel 2 ELISA Kit; Vanilloid receptor like protein 2 ELISA Kit; Vanilloid receptor related osmotically activated channel ELISA Kit; Vanilloid receptor-like channel 2 ELISA Kit; Vanilloid receptor-like protein 2 ELISA Kit; Vanilloid receptor-related osmotically-activated channel ELISA Kit; VR 4 ELISA Kit; VR OAC ELISA Kit; VR-OAC ELISA Kit; VR4 ELISA Kit; VRL 2 ELISA Kit; VRL-2 ELISA Kit; VRL2 ELISA Kit; VROAC ELISA Kit

缩写

TRPV4

Uniprot No

Q9HBA0

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TrpV4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		101
		Range %		95-108
1:2		Average %		85
		Range %		80-93
1:4		Average %		92
		Range %		88-97
1:8		Average %		90
		Range %		85-94

回收率

The recovery of human TrpV4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		94-103
EDTA plasma (n=4)		94		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1000 2.004 1.925 1.965 1.840 500 1.682 1.601 1.642 1.517 250 1.350 1.410 1.380 1.255 125 1.023 1.052 1.038 0.913 62.5 0.671 0.703 0.687 0.562 31.25 0.487 0.456 0.472 0.347 15.6 0.272 0.264 0.268 0.143 0 0.128 0.121 0.125

货期

3-5 working days