产品描述
This Mouse SIRT1 ELISA Kit was designed for the quantitative measurement of Mouse SIRT1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
Sirt1 ELISA Kit; Sir2l1 ELISA Kit; NAD-dependent protein deacetylase sirtuin-1 ELISA Kit; EC 2.3.1.286 ELISA Kit; NAD-dependent protein deacylase sirtuin-1 ELISA Kit; EC 2.3.1.- ELISA Kit; Regulatory protein SIR2 homolog 1 ELISA Kit; SIR2-like protein 1 ELISA Kit; SIR2alpha ELISA Kit; Sir2 ELISA Kit; mSIR2a) [Cleaved into: SirtT1 75 kDa fragment ELISA Kit; 75SirT1)] ELISA Kit
缩写
SIRT1
Uniprot No
Q923E4
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Epigenetics and Nuclear Signaling
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Sirt1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-95 |
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| 1:2 |
Average % |
93 |
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| Range % |
88-98 |
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| 1:4 |
Average % |
95 |
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| Range % |
89-100 |
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| 1:8 |
Average % |
97 |
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| Range % |
93-103 |
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回收率
| The recovery of mouse Sirt1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
95-104 |
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| EDTA plasma (n=4) |
98 |
92-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.322 |
2.162 |
2.242 |
2.071 |
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| 5 |
1.516 |
1.447 |
1.482 |
1.311 |
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| 2.5 |
0.897 |
0.834 |
0.866 |
0.695 |
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| 1.25 |
0.512 |
0.509 |
0.511 |
0.340 |
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| 0.625 |
0.349 |
0.327 |
0.338 |
0.167 |
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| 0.312 |
0.278 |
0.258 |
0.268 |
0.097 |
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| 0.156 |
0.232 |
0.219 |
0.226 |
0.055 |
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| 0 |
0.175 |
0.167 |
0.171 |
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货期
3-5 working days