产品描述

This Human SEMA7A ELISA Kit was designed for the quantitative measurement of Human SEMA7A protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

CD 108 ELISA Kit; CD108 ELISA Kit; CD108 antigen ELISA Kit; CDw108 ELISA Kit; H Sema K1 ELISA Kit; H Sema L ELISA Kit; H-SEMA-K1 ELISA Kit; H-Sema-L ELISA Kit; JMH ELISA Kit; JMH blood group antigen ELISA Kit; John Milton Hagen blood group H Sema K1 ELISA Kit; John Milton Hargen human blood group Ag ELISA Kit; John-Milton-Hargen human blood group Ag ELISA Kit; MGC126692 ELISA Kit; MGC126696 ELISA Kit; SEM7A_HUMAN ELISA Kit; Sema domain immunoglobulin domain (Ig) and GPI membrane anchor 7A ELISA Kit; Sema K1 ELISA Kit; Sema L ELISA Kit; SEMA7A ELISA Kit; SEMAK1 ELISA Kit; SEMAL ELISA Kit; Semaphorin 7A GPI membrane anchor ELISA Kit; Semaphorin K1 ELISA Kit; Semaphorin L ELISA Kit; Semaphorin-7A ELISA Kit; Semaphorin-K1 ELISA Kit; Semaphorin-L ELISA Kit; Semaphorin7a ELISA Kit

缩写

SEMA7A

Uniprot No

O75326

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SEMA7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		87-97
1:2		Average %		92
		Range %		82-96
1:4		Average %		94
		Range %		84-98
1:8		Average %		87
		Range %		82-92

回收率

The recovery of human SEMA7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		83-94
EDTA plasma (n=4)		103		98-107

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.246 2.089 2.168 1.984 10 1.596 1.692 1.644 1.460 5 1.123 1.068 1.096 0.912 2.5 0.699 0.687 0.693 0.509 1.25 0.465 0.454 0.460 0.276 0.625 0.339 0.324 0.332 0.148 0.312 0.260 0.268 0.264 0.080 0 0.185 0.183 0.184

货期

3-5 working days