产品描述
PF4,全称Platelet Factor 4,是一种由血小板分泌的蛋白质,属于趋化因子家族。它参与了血小板凝集、炎症反应、血管生成等生物学过程。在血液疾病、炎症、心血管疾病等多种疾病中,其含量都可能发生改变。PF4通过结合一些分子来发挥其生物学作用,如绑定血小板表面受体、促进血管生成等。PF4的水平可能受到肿瘤、炎症、感染、创伤等因素的影响。PF4在人类血浆中的正常含量为4-80 ng/mL,可以通过ELISA等方法进行检测。
华美生物所提供的Human Platelet Factor 4,PF-4 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的PF4,其灵敏度为1.56 ng/mL,检测范围为6.25 ng/mL-400 ng/mL。
别名
C-X-C motif chemokine 4 ELISA Kit; Chemokine (C X C motif) ligand 4 ELISA Kit; Chemokine (CXC motif) ligand 4 ELISA Kit; chemokine ligand 4 ELISA Kit; CXCL 4 ELISA Kit; CXCL4 ELISA Kit; Iroplact ELISA Kit; MGC138298 ELISA Kit; Oncostatin A ELISA Kit; Oncostatin-A ELISA Kit; OncostatinA ELISA Kit; PF 4 ELISA Kit; PF-4 ELISA Kit; PF4 ELISA Kit; Pf4a ELISA Kit; Platelet factor 4 ELISA Kit; PLF4_HUMAN ELISA Kit; SCYB 4 ELISA Kit; SCYB4 ELISA Kit; short form ELISA Kit; Small inducible cytokine subfamily B ELISA Kit; Small inducible cytokine subfamily member 4 ELISA Kit
缩写
PF4
Uniprot No
P02776
灵敏度
1.56 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
95 |
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| Range % |
92-101 |
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| 1:40 |
Average % |
93 |
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| Range % |
83-99 |
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| 1:80 |
Average % |
95 |
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| Range % |
90-100 |
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回收率
| The recovery of human PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
86-97 |
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| EDTA plasma (n=4) |
96 |
90-101 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.548 |
2.452 |
2.500 |
2.294 |
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| 200 |
2.196 |
2.076 |
2.136 |
1.930 |
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| 100 |
1.632 |
1.593 |
1.613 |
1.407 |
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| 50 |
1.082 |
1.122 |
1.102 |
0.896 |
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| 25 |
0.675 |
0.645 |
0.660 |
0.454 |
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| 12.5 |
0.441 |
0.436 |
0.439 |
0.233 |
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| 6.25 |
0.320 |
0.315 |
0.318 |
0.112 |
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| 0 |
0.205 |
0.207 |
0.206 |
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货期
3-5 working days