产品描述
This Mouse TCC C5b9 ELISA Kit was designed for the quantitative measurement of Mouse TCC C5b9 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL .
缩写
TCC C5b9
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 88 | |
| Range % | 84-96 | |
| 1:200 | Average % | 98 | |
| Range % | 91-105 | |
| 1:400 | Average % | 95 | |
| Range % | 91-99 | |
| 1:800 | Average % | 92 | |
| Range % | 88-96 | |
回收率
| The recovery of mouse TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 87-97 | |
| EDTA plasma (n=4) | 97 | 92-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 50 | 2.684 | 2.810 | 2.747 | 2.596 | | | 25 | 2.054 | 2.212 | 2.133 | 1.982 | | | 12.5 | 1.426 | 1.446 | 1.436 | 1.285 | | | 6.25 | 0.854 | 0.867 | 0.861 | 0.710 | | | 3.12 | 0.622 | 0.585 | 0.604 | 0.453 | | | 1.56 | 0.337 | 0.350 | 0.344 | 0.193 | | | 0.78 | 0.258 | 0.264 | 0.261 | 0.110 | | | 0 | 0.148 | 0.154 | 0.151 | | |
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货期
3-5 working days