产品描述
This Human UCP1 ELISA Kit was designed for the quantitative measurement of Human UCP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .
别名
mitochondrial brown fat uncoupling protein ELISA Kit; Mitochondrial brown fat uncoupling protein 1 ELISA Kit; SLC25A7 ELISA Kit; Solute carrier family 25 member 7 ELISA Kit; Thermogenin ELISA Kit; UCP 1 ELISA Kit; UCP ELISA Kit; UCP1 ELISA Kit; UCP1_HUMAN ELISA Kit; uncoupling protein 1 (mitochondrial, proton carrier) ELISA Kit; Uncoupling protein 1 ELISA Kit
缩写
UCP1
Uniprot No
P25874
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human UCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 87-99 | |
| 1:2 | Average % | 101 | |
| Range % | 93-105 | |
| 1:4 | Average % | 94 | |
| Range % | 86-98 | |
| 1:8 | Average % | 98 | |
| Range % | 93-105 | |
回收率
| The recovery of human UCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 90-98 | |
| EDTA plasma (n=4) | 92 | 89-95 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.093 | 1.952 | 2.023 | 1.940 | | | 1000 | 1.344 | 1.321 | 1.333 | 1.250 | | | 500 | 0.781 | 0.777 | 0.779 | 0.696 | | | 250 | 0.471 | 0.455 | 0.463 | 0.380 | | | 125 | 0.283 | 0.278 | 0.281 | 0.198 | | | 62.5 | 0.212 | 0.207 | 0.210 | 0.127 | | | 31.25 | 0.149 | 0.145 | 0.147 | 0.064 | | | 0 | 0.085 | 0.081 | 0.083 | | |
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货期
3-5 working days