产品描述

This human SOD2 ELISA Kit is suitable for qualitatively determining human concentrations in serum, plasma, cell culture supernates, cell lysates, or tissue homogenates in vitro. SOD2 is a mitochondrial-specific antioxidant enzyme that dismutates superoxide to hydrogen peroxide, which is then converted to water by catalase and glutathione peroxidase. SOD2 is synthesized in the cytoplasm and localizes to the mitochondrial matrix, where it scavenges and accelerates the dismutation of superoxide anion generated by respiratory chain enzymes. It also plays a crucial role in the maintenance of vascular function. SOD2 overexpression and excess generation of hydrogen peroxide have been involved in angiogenesis by enhancing oxidation of phosphatase and tensin homolog deleted from chromosome 10, cell differentiation through prolonged ERK1/2 signaling, as well as pulmonary hypertension through the activation of hypoxia-inducible factor 1α-O2–sensitive pathways. A deficiency of SOD2 in apolipoprotein E–deficient mice leads to mitochondrial damage and impaired vascular relaxation in response to acetylcholine.

This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human SOD2 in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-human SOD2 antibody. Biotin-labeled SOD2 antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the SOD2 in the human samples. SOD2 binds to the biotinylated anti-human human monoclonal antibody. And the biotin on the biotinylated anti-SOD2 human monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of SOD2 in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

别名

Indophenoloxidase B ELISA Kit; IPO B ELISA Kit; IPOB ELISA Kit; Manganese containing superoxide dismutase ELISA Kit; Manganese SOD ELISA Kit; Manganese superoxide dismutase ELISA Kit; Mangano superoxide dismutase ELISA Kit; Mn SOD ELISA Kit; Mn superoxide dismutase ELISA Kit; MNSOD ELISA Kit; MVCD6 ELISA Kit; SOD 2 ELISA Kit; SOD2 ELISA Kit; SODM_HUMAN ELISA Kit; Superoxide dismutase [Mn] mitochondrial ELISA Kit; Superoxide dismutase [Mn], mitochondrial ELISA Kit; Superoxide dismutase 2 mitochondrial ELISA Kit

缩写

SOD2

Uniprot No

P04179

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SOD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:200		Average %		88
		Range %		80-92
1:400		Average %		95
		Range %		91-100
1:800		Average %		100
		Range %		90-110
1:1600		Average %		93
		Range %		86-98

回收率

The recovery of human SOD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-98
EDTA plasma (n=4)		93		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.248 2.152 2.200 2.107 500 1.967 1.890 1.929 1.836 250 1.685 1.604 1.645 1.552 125 1.334 1.319 1.327 1.234 62.5 0.925 0.904 0.915 0.822 31.2 0.629 0.590 0.610 0.517 15.6 0.277 0.269 0.273 0.180 0 0.096 0.090 0.093

货期

3-5 working days