产品描述
This Human sPECAM1/sCD31 ELISA Kit was designed for the quantitative measurement of Human sPECAM1/sCD31 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31
缩写
sPECAM1/sCD31
Uniprot No
P16284
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human SPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:200 |
Average % |
88 |
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| Range % |
84-93 |
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| 1:400 |
Average % |
100 |
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| Range % |
95-110 |
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| 1:800 |
Average % |
90 |
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| Range % |
85-97 |
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回收率
| The recovery of human SPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
84 |
80-90 |
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| EDTA plasma (n=4) |
93 |
89-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.468 |
2.324 |
2.396 |
2.302 |
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| 1000 |
1.931 |
1.852 |
1.892 |
1.798 |
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| 500 |
1.224 |
1.176 |
1.200 |
1.106 |
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| 250 |
0.568 |
0.602 |
0.585 |
0.491 |
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| 125 |
0.333 |
0.341 |
0.337 |
0.243 |
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| 62.5 |
0.237 |
0.230 |
0.234 |
0.140 |
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| 31.25 |
0.166 |
0.162 |
0.164 |
0.070 |
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| 0 |
0.093 |
0.094 |
0.094 |
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货期
3-5 working days