产品描述
This Human Surv Ab ELISA Kit was designed for the quantitative measurement of Human Surv Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
缩写
Surv Ab
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human anti-surv antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
81-90 |
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| 1:2 |
Average % |
96 |
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| Range % |
90-105 |
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| 1:4 |
Average % |
86 |
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| Range % |
81-90 |
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| 1:8 |
Average % |
93 |
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| Range % |
85-100 |
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回收率
| The recovery of human anti-surv antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
90-101 |
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| EDTA plasma (n=4) |
87 |
82-92 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.213 |
2.157 |
2.185 |
2.014 |
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| 20 |
1.612 |
1.512 |
1.562 |
1.391 |
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| 10 |
1.156 |
1.113 |
1.135 |
0.964 |
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| 5 |
0.779 |
0.735 |
0.757 |
0.586 |
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| 2.5 |
0.522 |
0.492 |
0.507 |
0.336 |
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| 1.25 |
0.383 |
0.366 |
0.375 |
0.204 |
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| 0.625 |
0.267 |
0.283 |
0.275 |
0.104 |
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| 0 |
0.168 |
0.173 |
0.171 |
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货期
3-5 working days