产品描述
This Mouse TNF-α Ab ELISA Kit was designed for the quantitative measurement of Mouse TNF-α Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
antibody for DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
缩写
TNF-α Ab
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-α antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
97 |
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| Range % |
90-105 |
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| 1:4 |
Average % |
87-96 |
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| Range % |
82-96 |
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| 1:8 |
Average % |
93 |
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| Range % |
88-100 |
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回收率
| The recovery of mouse TNF-α antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
85-94 |
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| EDTA plasma (n=4) |
92 |
87-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.713 |
2.752 |
2.733 |
2.580 |
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| 5 |
2.212 |
2.349 |
2.281 |
2.128 |
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| 2.5 |
1.514 |
1.623 |
1.569 |
1.416 |
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| 1.25 |
0.849 |
0.864 |
0.857 |
0.704 |
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| 0.625 |
0.502 |
0.525 |
0.514 |
0.361 |
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| 0.312 |
0.313 |
0.316 |
0.315 |
0.162 |
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| 0.156 |
0.257 |
0.270 |
0.264 |
0.111 |
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| 0 |
0.148 |
0.157 |
0.153 |
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货期
3-5 working days