产品描述

This Human PRDX4 ELISA Kit was designed for the quantitative measurement of Human PRDX4 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

别名

Antioxidant enzyme 372 ELISA Kit; Antioxidant enzyme AOE372 ELISA Kit; AOE37 2 ELISA Kit; AOE37-2 ELISA Kit; AOE372 ELISA Kit; EC 1.11.1.15 ELISA Kit; Peroxiredoxin IV ELISA Kit; Peroxiredoxin-4 ELISA Kit; Peroxiredoxin4 ELISA Kit; PRDX 4 ELISA Kit; Prdx4 ELISA Kit; PRDX4_HUMAN ELISA Kit; PRX 4 ELISA Kit; Prx IV ELISA Kit; Prx-IV ELISA Kit; PRX4 ELISA Kit; PrxIV ELISA Kit; Thioredoxin dependent peroxide reductase A0372 ELISA Kit; Thioredoxin Peroxidase (Antioxidant Enzyme) ELISA Kit; Thioredoxin peroxidase ELISA Kit; Thioredoxin peroxidase AO372 ELISA Kit; Thioredoxin-dependent peroxide reductase A0372 ELISA Kit; TRANK ELISA Kit

缩写

PRDX4

Uniprot No

Q13162

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PRDX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		84-93
1:2		Average %		104
		Range %		100-108
1:4		Average %		83
		Range %		80-86
1:8		Average %		94
		Range %		91-97

回收率

The recovery of human PRDX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		85		81-89
EDTA plasma (n=4)		92		86-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 40 2.648 2.609 2.629 2.459 20 2.224 2.298 2.261 2.091 10 1.545 1.604 1.575 1.405 5 0.816 0.835 0.826 0.656 2.5 0.531 0.501 0.516 0.346 1.25 0.325 0.317 0.321 0.151 0.625 0.241 0.260 0.251 0.081 0 0.169 0.171 0.170

货期

3-5 working days