产品描述

This Human SERPING1 ELISA Kit was designed for the quantitative measurement of Human SERPING1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 18.75 ng/mL-1200 ng/mL and the sensitivity is 4.7 ng/mL.

别名

C1 esterase inhibitor ELISA Kit; C1 Inh ELISA Kit; C1 inhibiting factor ELISA Kit; C1 inhibitor ELISA Kit; C1-inhibiting factor ELISA Kit; C1IN ELISA Kit; C1Inh ELISA Kit; C1NH ELISA Kit; complement component 1 inhibitor ELISA Kit; esterase inhibitor ELISA Kit; HAE1 ELISA Kit; HAE2 ELISA Kit; IC1_HUMAN ELISA Kit; Plasma protease C1 inhibitor ELISA Kit; Serine (or cysteine) proteinase inhibitor clade G member 1 ELISA Kit; serine/cysteine proteinase inhibitor clade G member 1 ELISA Kit; Serpin G1 ELISA Kit; serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 ELISA Kit; SERPING1 ELISA Kit

缩写

SERPING1

Uniprot No

P05155

灵敏度

4.7 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Blood Coagulation

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SERPING1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:200		Average %		99
		Range %		95-103
1:400		Average %		94
		Range %		91-98
1:800		Average %		95
		Range %		90-100
1:1600		Average %		92
		Range %		85-96

回收率

The recovery of human SERPING1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		88-99
EDTA plasma (n=4)		89		82-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average     1200 0.090 0.091 0.091     600 0.115 0.117 0.116     300 0.166 0.175 0.171     150 0.272 0.261 0.267     75 0.466 0.455 0.461     37.5 0.784 0.773 0.779     18.75 1.020 1.110 1.065     0 2.103 2.205 2.154

货期

3-5 working days