产品描述

This Human REG4 ELISA Kit was designed for the quantitative measurement of Human REG4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

Gastrointestinal secretory protein ELISA Kit; GISP ELISA Kit; Reg IV ELISA Kit; REG like protein ELISA Kit; REG-4 ELISA Kit; REG-like protein ELISA Kit; Reg4 ELISA Kit; REG4_HUMAN ELISA Kit; Regenerating gene type IV ELISA Kit; Regenerating islet derived family member 4 ELISA Kit; Regenerating islet derived protein 4 precursor ELISA Kit; Regenerating islet-derived protein 4 ELISA Kit; Regenerating islet-derived protein IV ELISA Kit; REGIV ELISA Kit; RELP ELISA Kit

缩写

REG4

Uniprot No

Q9BYZ8

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human REG-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		87
		Range %		83-92
1:2		Average %		92
		Range %		90-95
1:4		Average %		96
		Range %		93-99
1:8		Average %		95
		Range %		88-98

回收率

The recovery of human REG-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		89-94
EDTA plasma (n=4)		92		90-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.832 2.801 2.817 2.676 10 2.238 2.187 2.213 2.072 5 1.419 1.385 1.402 1.261 2.5 0.673 0.654 0.664 0.523 1.25 0.434 0.411 0.423 0.282 0.625 0.281 0.269 0.275 0.134 0.312 0.211 0.202 0.207 0.066 0 0.143 0.139 0.141

货期

3-5 working days