产品描述
This Human PDGF-CC ELISA Kit was designed for the quantitative measurement of Human PDGF-CC protein in serum, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
缩写
PDGF-CC
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 88-96 | |
| 1:2 | Average % | 95 | |
| Range % | 92-99 | |
| 1:4 | Average % | 94 | |
| Range % | 89-101 | |
| 1:8 | Average % | 89 | |
| Range % | 84-95 | |
回收率
| The recovery of human PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 91-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.308 | 2.418 | 2.363 | 2.260 | | | 5 | 1.887 | 2.032 | 1.960 | 1.857 | | | 2.5 | 1.452 | 1.423 | 1.438 | 1.335 | | | 1.25 | 0.782 | 0.821 | 0.802 | 0.699 | | | 0.625 | 0.477 | 0.497 | 0.487 | 0.384 | | | 0.312 | 0.325 | 0.346 | 0.336 | 0.233 | | | 0.156 | 0.215 | 0.205 | 0.210 | 0.107 | | | 0 | 0.104 | 0.101 | 0.103 | | | | | | | | | | |
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货期
3-5 working days