产品描述
This Rabbit SDF-1/CXCL12 ELISA Kit was designed for the quantitative measurement of Rabbit SDF-1/CXCL12 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.57 ng/mL-10 ng/mL and the sensitivity is 0.36 pg/mL.
缩写
SDF-1/CXCL12
灵敏度
0.125 ng/ml.
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SDF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
102 |
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| Range % |
88-106 |
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| 1:2 |
Average % |
95 |
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| Range % |
89-102 |
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| 1:4 |
Average % |
88 |
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| Range % |
85-94 |
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| 1:8 |
Average % |
99 |
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| Range % |
93-104 |
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回收率
| The recovery of rabbit SDF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
104 |
100-108 |
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| EDTA plasma (n=4) |
93 |
91-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.167 |
1.999 |
2.083 |
2.046 |
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| 5.71 |
1.154 |
1.074 |
1.114 |
1.077 |
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| 2.28 |
0.399 |
0.384 |
0.392 |
0.355 |
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| 1.14 |
0.211 |
0.212 |
0.212 |
0.175 |
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| 0.57 |
0.114 |
0.111 |
0.113 |
0.076 |
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| 0 |
0.035 |
0.038 |
0.037 |
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货期
3-5 working days