产品描述

This Human TTN ELISA Kit was designed for the quantitative measurement of Human TTN protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL .

别名

MPRM ELISA Kit; Cardiomyopathy dilated 1G (autosomal dominant) ELISA Kit; CMD1G ELISA Kit; CMH 9 ELISA Kit; CMH9 ELISA Kit; CMPD 4 ELISA Kit; CMPD4 ELISA Kit; Connectin ELISA Kit; DKFZp451N061 ELISA Kit; EOMFC ELISA Kit; FLJ26020 ELISA Kit; FLJ26409 ELISA Kit; FLJ32040 ELISA Kit; FLJ34413 ELISA Kit; FLJ39564 ELISA Kit; FLJ43066 ELISA Kit; HMERF ELISA Kit; LGMD2J ELISA Kit; MU RMS 40.14 ELISA Kit; MYLK5 ELISA Kit; Rhabdomyosarcoma antigen ELISA Kit; Rhabdomyosarcoma antigen MU RMS 40.14 ELISA Kit; Rhabdomyosarcoma antigen MU-RMS-40.14 ELISA Kit; Titin ELISA Kit; TITIN_HUMAN ELISA Kit; TMD ELISA Kit; TTN ELISA Kit

缩写

TTN

Uniprot No

Q8WZ42

灵敏度

5.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		98
		Range %		94-104
1:2		Average %		96
		Range %		91-101
1:4		Average %		93
		Range %		87-99
1:8		Average %		92
		Range %		84-98

回收率

The recovery of human TTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		92-100
EDTA plasma (n=4)		98		90-106

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   1500 2.195 2.095 2.145 2.014   750 1.594 1.494 1.544 1.413   375 0.932 0.892 0.912 0.781   187.5 0.506 0.486 0.496 0.365   94 0.352 0.342 0.347 0.216   47 0.229 0.219 0.224 0.093   23.5 0.168 0.162 0.165 0.034   0 0.131 0.130 0.131

货期

3-5 working days