产品描述
The Rat HSP47/Serpin H1 ELISA Kit is a high-quality research tool designed for the quantitative detection of Serpin H1 in rat serum, plasma, tissue homogenates, and cell lysates. This kit is specifically designed for use in neuroscience research and can detect levels of Serpin H1 as low as 0.039 ng/mL with a detection range of 0.156 ng/mL to 10 ng/mL.
The assay principle is based on the sandwich technique, in which the capture antibody is pre-coated on a microplate and the detection antibody is labeled with biotin. Upon binding to the target analyte, the complex is captured by streptavidin-HRP conjugate and the substrate TMB is added to produce a colored reaction product that is quantified by measuring the absorbance at 450 nm.
With an assay time of 1-5 hours, a sample volume of 50-100 µl, and a detection wavelength of 450 nm, the HSP47 ELISA kit provides accurate and reliable results for your research needs. The kit includes all necessary components for the assay, such as microplate wells, wash buffer, detection antibody, and standards.
别名
Serpinh1 ELISA Kit; Cbp1 ELISA Kit; Hsp47 ELISA Kit; Serpin H1 ELISA Kit; 47 kDa heat shock protein ELISA Kit; Collagen-binding protein ELISA Kit; Colligin ELISA Kit; GP46 ELISA Kit
缩写
SERPINH1
Uniprot No
P29457
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HSP47 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 81-93 | |
| 1:2 | Average % | 102 | |
| Range % | 98-105 | |
| 1:4 | Average % | 92 | |
| Range % | 88-96 | |
| 1:8 | Average % | 95 | |
| Range % | 91-99 | |
回收率
| The recovery of rat HSP47 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 89-96 | |
| EDTA plasma (n=4) | 87 | 83-92 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.311 | 2.507 | 2.409 | 2.261 | | | 5 | 1.740 | 1.645 | 1.693 | 1.545 | | | 2.5 | 1.087 | 1.124 | 1.106 | 0.958 | | | 1.25 | 0.664 | 0.652 | 0.658 | 0.510 | | | 0.625 | 0.420 | 0.433 | 0.427 | 0.279 | | | 0.312 | 0.339 | 0.327 | 0.333 | 0.185 | | | 0.156 | 0.226 | 0.230 | 0.228 | 0.080 | | | 0 | 0.147 | 0.148 | 0.148 | | |
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货期
3-5 working days
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