产品描述
This Human PG I ELISA Kit was designed for the quantitative measurement of Human PG I protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.
缩写
PG I
灵敏度
1.56 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human pepsinogen I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 84 | |
| Range % | 80-92 | |
| 1:2 | Average % | 106 | |
| Range % | 100-115 | |
| 1:4 | Average % | 97 | |
| Range % | 94-112 | |
| 1:8 | Average % | 93 | |
| Range % | 86-98 | |
回收率
| The recovery of human pepsinogen I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-99 | |
| EDTA plasma (n=4) | 97 | 90-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.396 | 2.252 | 2.324 | 2.207 | | | 200 | 1.612 | 1.736 | 1.674 | 1.557 | | | 100 | 1.104 | 1.054 | 1.079 | 0.962 | | | 50 | 0.662 | 0.639 | 0.651 | 0.534 | | | 25 | 0.399 | 0.424 | 0.412 | 0.295 | | | 12.5 | 0.292 | 0.309 | 0.301 | 0.184 | | | 6.25 | 0.205 | 0.199 | 0.202 | 0.085 | | | 0 | 0.118 | 0.115 | 0.117 | | |
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货期
3-5 working days