产品描述
TNFRSF11B(Tumor Necrosis Factor Receptor Superfamily Member 11B),也被称为骨保护素(Osteoprotegerin, OPG),是一种可溶性受体蛋白。它通过结合受体激活因子κB配体(RANKL)来抑制骨吸收过程,从而在骨代谢中发挥关键作用。此外,OPG还参与血管钙化、炎症和免疫功能等其他生物学过程。OPG的含量是调节骨吸收和形成的重要因素。在某些疾病中,OPG的过量或缺乏会导致骨疾病的发生,如骨质疏松症和肿瘤性骨病变。
华美生物所提供的Mouse Osteoprotegerin,OPG ELISA KIT属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的TNFRSF11B,其灵敏度为3.9 pg/mL,检测范围为15.6 pg/mL-1000 pg/mL。
别名
Tnfrsf11b ELISA Kit; Ocif ELISA Kit; Opg ELISA Kit; Tumor necrosis factor receptor superfamily member 11B ELISA Kit; Osteoclastogenesis inhibitory factor ELISA Kit; Osteoprotegerin ELISA Kit
缩写
TNFRSF11B
Uniprot No
O08712
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse OPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
87 |
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| Range % |
83-90 |
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| 1:10 |
Average % |
102 |
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| Range % |
98-106 |
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| 1:20 |
Average % |
85 |
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| Range % |
81-89 |
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| 1:40 |
Average % |
97 |
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| Range % |
93-100 |
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回收率
| The recovery of mouse OPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
85 |
81-89 |
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| EDTA plasma (n=4) |
96 |
92-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.045 |
2.102 |
2.074 |
1.938 |
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| 500 |
1.357 |
1.308 |
1.333 |
1.197 |
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| 250 |
0.879 |
0.915 |
0.897 |
0.761 |
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| 125 |
0.593 |
0.578 |
0.586 |
0.450 |
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| 62.5 |
0.381 |
0.368 |
0.375 |
0.239 |
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| 31.2 |
0.275 |
0.298 |
0.287 |
0.151 |
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| 15.6 |
0.193 |
0.187 |
0.190 |
0.054 |
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| 0 |
0.135 |
0.137 |
0.136 |
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货期
3-5 working days